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Table 1 Mutations isolated. Summary of complementation analysis and phenotypic classes identified. Lines were placed into complementation groups following pairwise testing of all mutations identified. The gene affected is listed where known; otherwise the smallest deficiency or combination of deficiencies uncovering the mutation is shown together with the cytological region. Where we have only one allele in a complementation group which fails to complement multiple regions all lethal deficiencies are listed. Notes: iIdentified in our pupal lethal screen. iiMapped by recombination with rucuca chromosome followed by clonal analysis.

From: A mosaic genetic screen for novel mutations affecting Drosophila neuroblast divisions

Group

Alleles

Gene/lethal deficiency

Cytology

Further mapping data/Comments

Asymmetric cell division defects

1

PL26i

Df(3R)p712

84D4;85B6

No phenotype is observed in PL26/Df(3R)p712 hemizygotes

6

PL17i

ida

  

13

J16

Not in deficiency kit

3L

Also carries a mutation in polo

8

D76

Not in deficiency kit

61F8;72D1ii

Lethality is caused by a mutation in trio which does not cause the Miranda phenotype

Cell division defects

 

Proliferation defects

2

LVC73i

Df(3L)GN24 or Df(3L)st-f13

63F4;64C15 or 72C1;73A4

 

3

PL13i

Df(3R)WIN11 or Df(3R)Dr-rv1

83E1;84A5 or 99A1;B11

 

6

PL17i

ida

  

7

A55, GL72

small-minded

  

8

D76

Df(3L)Ar14-8

61C5;62A8

Lethality in this region is caused by a mutation in trio

9

A38, B10, B18

Df(3L)Exel6112 + Df(3L)ED4408

66B5;66C5

 

10

A57

Df(3L)ZN47 or Df(3L)fz-GF3b

64C;65C or 70C1;D5

Phenotype maps to 70C1;70D5 regionii

11

C10

Df(3L)Ar14-8 or Df(3L)AC1

61C5;62A8 or 67A2;D13

Phenotype maps to 67A2;67D13 regionii

12

E45

makos

  
 

Chromosome separation defects

14

A9, A67, B14, H10, DL42

Klp61F

  

15

G34

Df(3l)ri-XT1

77E2;78A4

Complements Df(3L) ED4861, Df(3L)ME107

16

C93

separase

 

Hemizogotes show multiple crescents of Miranda

 

Multinucleate cells

5

D97, CMV111i, IV61i

sticky

  

17

C33

pebble

  

18

A59, H2, GL22, C22

Taf-4

  

19

A42

Df(3L)ED4858 + Df(3L)Exel6136

77B2;77C1

 

20

B27, H87

Df(3L)BSC13 + Df(3L)ED4408

66B12;66C5

 

21

C26, C36

Df(3L)XDI98

65A2;65E1

Complements Df(3L)ZN47 and Df(3L)BSC27

22

D7, CL89

Df(3L)GN34 + Df(3L)ED4341

63F6;64A9

Complements Df(3L)Exel6099

23

D24

Df(3L)Exel7253

73D5;73E4

 

24

D67, OL77

Df(3L)ri-XT1

77E2;78A4

Complements Df(3L)ED4861

25

D75

Df(3L)R-G7 or Df(3L)vin7 + Df(3L)eygC1or Df(3L)fz-M21 + Df(3L)XG-5

62B8;F5 or 69A4;B5 or 71C2;E5

 

26

F58

Df(3L)BSC33

65E10;65F6

 

27

GL45

Df(3L)Exel6105

64D1;64D6

 

28

H67, J2

Df(3L)Exel6087

62A2;62A7

Complements Df(3L)ED4238

29

D40

7 lethal deficiencies in 4 lethal regions

63C2;F7 or 65F3;F6 or 66B8;C5 or 66E1;E6

 

30

B55

Not in deficiency kit

3L

 

31

E47, GL26

Not in deficiency kit

3L

 
 

Vesicular/membrane defects

32

B44, C19, C62

Aats-ile

  

33

A69

Int6

  

34

D56

neurexin

 

Multinucleate cells are also observed with low frequency

35

E25, E55

reptin

  

36

B11, O29

Taf-6

  

37

A11, A572, A58, E80, O49

Df(3L)X-21.2

71F1;72A2

 

38

A44

Df(3L)GN24 or Df(3L)vin5 + Df(3L)vin7 or Df(3L)fz-M21

63F4;64C15 or 68C8;69A3 or 70D2;71E5

 

39

OL61

Df(3L)AC1

67A2;67D13

 

40

B29, H26

Df(3L)ED4858

76E1;76F1

Complements Df(3L)ED229 and Df(3L)ED4861

41

CMV45, ML72

Df(3L)rdgC-co2

77C6;77D1

Complements Df(3L)ED4858 and Df(3L)Exel6136

42

M7

Df(3L)66C-G28 or Df(3L)rdgC-co2 + Df(3L)ri-79c

66B8;C10 or 77B;D1

 

43

OL24

Df(3L)X-21.2

71F1;72A2

 

44

CL62

Df(3L)ED4858

76D3;77C1

 

45

F582, G82, ML72

Df(3L)Exel6132 + Df(3L)Exel9005

74B2;74D2

ML72 is also allelic to CMV45 (group 41)

46

GL29

Df(3L)ZP1 or Df(3L)ED218

66A17;C5 or 71B1;E1

Phenotype maps to 71B1;71E1 regionii; complements Df(3L)Exel6125

47

A41, E50

not in deficiency kit

3L

 

48

B57

n.d.

3L

 

4

C79

n.d.

3L

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