Figure 3

lin-3 mosaic analyis. (A) lin-3 mosaic animal at L4 stage with normal vulval development. Anchor cell labeled with arrowhead; bar indicates vulva. (B) Same lin-3 mosaic animal viewed under epifluoresence. The anchor cell has an intense spot of fluorescence, and thus the transgenic array (lin-3(+) + lacO + pPD118-33). The vulval descendants do not have the intense spot of fluorescence, and thus lack the transgenic array. (C) lin-3 mosaic animal at L4 stage with no Pn.px cells adopting vulval fate. Anchor cell labeled with arrowhead. Pn.px cell progeny labeled with arrows. (D) Same lin-3 mosaic animal viewed under epifluoresence. The anchor cell does not have the intense spot of fluorescence, and thus lacks the transgenic array (lin-3(+) + lacO + pPD118-33). The Pn.px cells do have the intense spot of fluorescence, and thus have the transgenic array. (E) lin-3 mosaic animal at L4 stage with multivulva phenotype. Anchor cell labeled with arrowhead; bar indicates vulva. (F) Same lin-3 mosaic animal viewed under epifluoresence. The anchor cell has an intense spot of fluorescence, and thus the transgenic array. The vulval descendants do not have the intense spot of fluorescence, and thus lack the transgenic array.