Figure 2
From: Loss of imprinting at the Dlk1-Gtl2 locus caused by insertional mutagenesis in the Gtl2 5' region
Expression levels of Dlk1 and Gtl2 mRNAs in Gtl2lacZ embryos. A) Representative Northern blots for Dlk1 and Gtl2 RNA levels, in comparison to βactin, in Gtl2lacZPat129 (left) and Gtl2lacZPatB 6(right) embryo and placenta. B) Graphical representation of quantitative expression data for Gtl2lacZPat129 (top) and Gtl2lacZPatB 6(bottom) embryos. In each graph, the black bars represent wild type (the mean of these values was set to 100), and the gray bars represent Gtl2lacZPat, E indicates embryo samples and P indicates placental samples; quantitative data is given for embryo only. P-values are indicated below each graph. (Top graph, Dlk1, WT, n = 3, Gtl2lacZPat129, n = 4; Gtl2, WT, n = 3, Gtl2lacZPat129, n = 4. Bottom graph, Dlk1, WT, n = 4, Gtl2lacZPatB 6, n = 4; Gtl2, WT, n = 4, Gtl2lacZPatB 6, n = 4). C) Representative Northern blots for Dlk1 and Gtl2 RNA levels, in comparison to β-actin, in Gtl2lacZMat129 (left) and Gtl2lacZMatB 6(right) embryos. D) Graphical representation of quantitative expression data for Gtl2lacZMat129 (top) and Gtl2lacZMatB 6(bottom) embryos. In each graph, the black bars represent wild type (the mean of these values was set to 100), and the gray bars represent Gtl2lacZMat. P-values are indicated below each graph. Differences in β-actin levels are the result of analyzing different midgestation embryonic stages. (Top graph, Dlk1, WT, n = 4, Gtl2lacZMat129, n = 4; Gtl2, WT, n = 4, Gtl2lacZMat129, n = 4. Bottom graph, Dlk1, WT, n = 4, Gtl2lacZMatB 6, n = 4; Gtl2, WT, n = 4, Gtl2lacZMatB 6, n = 4). All expression data includes embryos from at least two litters.