Figure 3

Dlk1 - Gtl2 imprinting analysis in Gtl2lacZ embryos. A) Imprinting assay for Dlk1 in wild type (left), Gtl2lacZPat¹²⁹ (center) and Gtl2lacZPat^{B 6}(right) embryos. B) Imprinting assay for Dlk1 in wild type (left), Gtl2lacZMat¹²⁹ (center) and Gtl2lacZMat^{B 6}(right) embryos. The Dlk1 polymorphism is an "A" in D embryos and a "G" in C embryos; the polymorphic base is boxed on each chromatogram. C) Imprinting assay for Gtl2 in Gtl2lacZPat¹²⁹ and Gtl2lacZPat^{B 6}embryos. D) Imprinting assay for Gtl2 in Gtl2lacZMat¹²⁹ and Gtl2lacZMat^{B 6}embryos. The Gtl2 polymorphism alters an Sfc I restriction enzyme site, with the site present in D and absent in C; the D and C bands are indicated at the right.