Dlk1 - Gtl2 imprinting analysis in Gtl2lacZ embryos. A) Imprinting assay for Dlk1 in wild type (left), Gtl2lacZPat129 (center) and Gtl2lacZPatB 6(right) embryos. B) Imprinting assay for Dlk1 in wild type (left), Gtl2lacZMat129 (center) and Gtl2lacZMatB 6(right) embryos. The Dlk1 polymorphism is an "A" in D embryos and a "G" in C embryos; the polymorphic base is boxed on each chromatogram. C) Imprinting assay for Gtl2 in Gtl2lacZPat129 and Gtl2lacZPatB 6embryos. D) Imprinting assay for Gtl2 in Gtl2lacZMat129 and Gtl2lacZMatB 6embryos. The Gtl2 polymorphism alters an Sfc I restriction enzyme site, with the site present in D and absent in C; the D and C bands are indicated at the right.