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Figure 5 | BMC Genetics

Figure 5

From: Loss of imprinting at the Dlk1-Gtl2 locus caused by insertional mutagenesis in the Gtl2 5' region

Figure 5

Expression analysis for Dlk1 - Gtl2 mRNA in Gtl2Δ5'Neo embryos. A) Representative Northern blots for Dlk1 and Gtl2 RNA levels, in comparison to β-actin, in Gtl2Δ5'NeoPat129 (left) and Gtl2Δ5'NeoPatB 6(right) embryos. B) Graphical representation of quantitative expression data for Gtl2Δ5'NeoPat129 (top) and Gtl2Δ5'NeoPatB 6(bottom) embryos. In each graph, the black bars represent wild type (the mean of these values was set to 100), and the gray bars represent Gtl2Δ5'NeoPat. P-values are indicated below each graph. (Top graph, Dlk1, WT, n = 4, Gtl2Δ5'NeoPat129, n = 6; Gtl2, WT, n = 4, Gtl2Δ5'NeoPat129, n = 6. Bottom graph, Dlk1, WT, n = 6, Gtl2Δ5'NeoPatB 6, n = 10; Gtl2, WT, n = 6, Gtl2Δ5'NeoPatB 6, n = 10). C) Representative Northern blots for Dlk1 and Gtl2 RNA levels, in comparison to β-actin, in Gtl2Δ5'NeoMat129 (left) and Gtl2Δ5'NeoMatB 6(right) embryos. D) Graphical representation of quantitative expression data for Gtl2Δ5'NeoMat129 (top) and Gtl2Δ5'NeoMatB 6(bottom) embryos. In each graph, the black bars represent wild type (the mean of these values was set to 100), and the gray bars represent Gtl2Δ5'NeoMat. P-values are indicated below each graph. (Top graph, Dlk1, WT, n = 4, Gtl2Δ5'NeoMat129, n = 6; Gtl2, WT, n = 4, Gtl2Δ5'NeoMat129, n = 6. Bottom graph, Dlk1, WT, n = 4, Gtl2Δ5'NeoMatB 6, n = 4; Gtl2, WT, n = 4, Gtl2Δ5'NeoMatB 6, n = 4).

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