Dlk1 - Gtl2 imprinting analysis in Gtl2Δ5'Neo embryos. A) Imprinting assay for Dlk1 in wild type (left) and Gtl2Δ5'NeoPat (right) embryos. B) Imprinting assay for Dlk1 in wild type (left), Gtl2Δ5'NeoMat129 (center) and Gtl2Δ5'NeoMatB 6(right) embryos. The Dlk1 polymorphism is an "A" in D embryos and a "G" in C embryos; the polymorphic base is boxed on each chromatogram. C) Imprinting assay for Gtl2 in Gtl2Δ5'NeoPat embryos. D) Imprinting assay for Gtl2 in Gtl2Δ5'NeoMat129 and Gtl2Δ5'NeoMatB 6embryos. The Gtl2 polymorphism alters an Sfc I restriction enzyme site, with the site present in D and absent in C; the D and C bands are indicated at the right.