Cellular localization and western blot analysis of wild type and fibronectin mutant forms of DmKal-1-Myc in COS-7 transfected cells. (A, B, C) Confocal analysis on unpermeabilized cells. DmKal-1-Myc proteins were detected by monoclonal 9E10 antibody. (A) DmKal-1-Myc is secreted from the transfected cells and bind to the cell surface. (B) DmKal-1-Myc [N236K] localizes on the cell surface of the transfected cells. (C) Dmkal-1-Myc [R159T+R161L] localizes on the cell surface of the transfected cells. (D) Western blot analysis of recombinant Dmkal-1-Myc proteins performed using the polyclonal ab9106-100 antibody. Recombinant DmKal-1-Myc proteins present in the cell extracts (cell extr.), in the wash with fresh medium (wash), in the wash with 100 μg/ml heparin (wash + hep.), and in the conditioned medium prior of the washes (medium). The intensity of the lower band varied in different experiments.