Evolutionary conservation of the heparan-sulfate binding site of the first FnIII domain. Amino acid sequence alignment of the first FnIII domain of different anosmin-1 proteins. An heparan-sulfate binding motif is conserved in the C-terminal part of the domain in all vertebrates, in C. elegans and in A. mellifera. In D. melanogaster, a complete heparan-sulfate binding motif is present at the N-terminus while, in A. mellifera, only a partially conserved motif is present in this region. In bold, the basic residues inside the motif. In the box, the asparagine at position 267 of the human protein and the equivalent amino acid in the other species.