Genotyping faecal samples of Bengal tiger Panthera tigris tigris for population estimation: A pilot study

BMC Genomic Data

Table 1 Polymorphic microsatellite markers that were screened on a panel of captive tigers (n = 21)

Locus	Primer Sequence (5'-3')	Repeat Type	T_m	k	Ho	He	PIC
D15	D15F: 5' TGTGACCTTTCTCTAGTTTC D15R: 5' GCACAAAACATTCAGTCTCC	(CA)₂₂, simple	51	8	0.381	0.740	0.689
3E6	3E6F: 5' CCTGGGGATAATAAAACTAGTA 2E6R: 5'CATGAATGAATCTTTACACTGA	(TAA)₁₁, simple	56	5	0.571	0.684	0.606
E21B	E21F: 5' GCGATAAAGGCTGGCAGAGG E21BR: 5' CTTTGAGGGTCTGTTCTACTGTGA	(CA)₂₁, simple	61	5	0.667	0.718	0.657
D10	D10F: 5' CCCTCTCTGTCCCTCCCTTG D10R: 5' GCCGTTTCCCTCATGCTACA	(GT)₁₄, simple	63	5	0.524	0.812	0.759
E7	E7F: 5'GCCCCAAAGCCCTAAAATAA E7R: 5'GCATGTCGGACAGTAAAGCA	(CA)₁₁CG(CA)₄, interrupted	55	7	0.571	0.662	0.600
C34	C34F: 5'CTCCACACTGAGCATGGAAA C34R: 5'CAACCAAAGGCAGGAACAGT	(CT)₂₁, simple	55	8	0.250	0.805	0.756
6HDZ003	Williamson et al (2002)		55	8	0.350	0.782	0.729
Fca304	Menotti-Raymond et al, 1999, Luo S-J et al, 2004		55	8	0.476	0.753	0.701
Fca43	Menotti-Raymond et al, 1999, Luo S-J et al, 2004		55	6	0.524	0.769	0.714
Fca139	Menotti-Raymond et al, 1999, Luo S-J et al, 2004		55	4	0.167	0.678	0.600

Tm = Annealing temperature; k = number of alleles; He = Expected heterozygosity; Ho = Observed Heterozygosity; PIC = Polymorphism Information Content

ISSN: 2730-6844