Figure 3
From: Assessment of genomic imprinting of SLC38A4, NNAT, NAP1L5, and H19 in cattle
Expression of NNAT and NAP1L5 transcripts obtained from cattle adult tissues and analyzed by RT-PCR. A, Expression analysis of NNAT transcripts. Lanes 1 and 13 are a 100-bp ladder marker. Lanes 2–11 are RT-PCR products obtained from adult ovary, endometrium, caruncle, heart, muscle, lung, liver, kidney, pancreas, and spleen, respectively. Lane 12 is a negative control. The sizes of RT-PCR products were 453 bp for transcript α (top bands), 370 bp for transcript β (middle bands), and 191 bp for b-actin (bottom bands). B, NAP1L5 transcripts amplified with NAP1L5-F/NAP1L5R. Lanes 1 and 13 are a 100-bp ladder marker. Lanes 2–11 are RT-PCR products obtained from adult ovary, endometrium, caruncle, heart, muscle, lung, liver, kidney, pancreas, and spleen, respectively. Lane 12 is a negative control. The bottom bands are RT-PCR products of b-actin.