Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) mutations associated with the domestic cat AB blood group

Table 2 Sequences and primers for CMAH analysis in the cat.

*CMAH* Region	Forward Primer 5' – 3'	Reverse Primer 5' – 3'	size bp
5'UTR – e1	TTCCTTTGCTGCTGGAGTG	CAACACTGAGCAAGCAGAGC	653
Exon 2	CAACACTGAGCAAGCAGAGC	GGTTTCCTCTCTCTCAGGGG	589
Exon 3	CTCTTAGGACATGGACTGAACG	CTTTGGTAAGACGGGTGAGAG	458
Exon 5	GTGTCTGTCGCATCACCTCT	GGATGAGAAGAAGCGGACTC	668
Exon 6	GGCAGAGAGCAGGAAGACAC	TTTTCCCCCATTTACCAATG	571
Exon 7	TAGCCGATTTTTGTCTGTGC	TCAACACTTCCATACATCACCC	669
Exon 8	GATCCCATCATGCTTCCTCT	GAAACCAGACTTGCAAAGCA	630
Exon 9	GTGTCTTTGGCTGGTTTGGT	GACTTTTAGCCACGCCATTC	642
Exon 11	GCCACACAGCTCCCATAGAC	TGTTGTTGTGGGTTTTGTGG	624
Exon 12	CATGGTGTTTGCTGTCTCAT	CCTCTACATGCTTACTGCCC	647
Exon 13	GACATGAGGGGTCGGTACG	CTCAACTCCAGCGTGCATTT	579
Exon 14	TTCACTGTTGGATGATGTGG	GACTTTCAGGGGAGTGAGAAG	700
¹cDNAe2-6	GACGACGGAGGTCTTGTTGT	TTACCCCACACTGAAGGAGC	608
¹cDNAe5-11	CAAGAGGATGGTGTTTGACC	GCATCATAGAGCCTCCAGAG	817
¹cDNAe9-14	GGAATCTCACCCATCAGACA	CTGTGGGTAGAGCAGACCTG	656
5' RACE		AAATACGTCAATCCCCCAGGCAGCTTCT	~500
3' RACE	AACTCCCTCTCACGCCACCCAACTG		440
Δ-53	TCCTCCGTCTCATACTTTGTGG	CATTTCCAGGGGAGCAGC	167
UTR SNPs	²GCCGGGTGTAAGGTTCTC	CACCTGGAGCCCAATAGG	247
³C-371T		AGCACGTGTAGGCT
³G-217A		AAGTGTGCAGGTG
Exon 2	²CTGCTGACCTGAAGGAAGGAAT	ACCCGTGAGAAGGCTCAGAA	187
³G139A		GGCGTGCAAGAACG
Exon 3	TGTCAGATGCACAAAGCACAAC	²TGCTGTGATCGTTACTGGCGT	141
³T265A	TGTGAGCACCATGAAA
Exon 10	CGACTAACTGTCACCTTTGTGTCTT	²TGAATGCTGCTGTAGGAGATGA	128
³G1266A	AAGCAAGGGCATCATA
Exon 12	²ATTCGGAGCCGGGTTGAC	CCTGTAAGTTGGAAAGTGCCTG	135
³G1591A		GTATATAGGATTCCAAACCC

1. Nested primers for the cDNA analysis. The positions of the primer in the exons are indicated in the primer name. 2. The M13 tail was attached to the indicated end of the indicated primer. 3. Primer for the pyrosequencing reaction of each amplified SNP region.

ISSN: 2730-6844