Relative luciferase activity of the wild type promoter (WT) and a promoter construct bearing the -60 C/T mutation in homozygosis. (A): relative luciferase activity in HUT78 cells following transfection of the promoter constructs by electroporation. Data shown is representative of three independent experiments. (B): relative luciferase activity in primary T-cells that were transfected with the promoter constructs using Amaxa transfection technology. Two independent experiments (exp.) were performed. In each experiment primary T-cells from 13 donors were used. Shown are results from donor #2 and donor #9. No differences between WT and mutant promoter activity were observed in the remaining 11 donors. Error bars represent the SEM of triplicates. Statistic analysis was performed applying unpaired t-test. Values < 0.05 were considered significant (* = p < 0.05, ** = p < 0.01).