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Table 3A Primers designed to screen the coding sequence of ENaC α, β, and γ genes. Optimum temperatures (°C) for PCR and DHPLC are included in the table.

From: Sequence analysis of coding and 3' and 5' flanking regions of the epithelial sodium channel α, β, and γ genes in Dahl S versus R rats

  

Sequence

PCR

DHPLC

ENaC α

    

Exon 1

Forward

GGTAGCACGGAGCTGGAG

64

63

 

Reverse

CCCAGGCTGAGTTCACTCTC

  
 

Forward

AGCTCAATACTGCTTGGTTGG

61

63

 

Reverse

GAAGAGCTCCCGGTAGGAG

  
 

Forward

GGGACAAACGTGAAGAGCAG

61

63

 

Reverse

CTTGCTTTTGTGCTGCTGAG

  

Exon 2

Forward

CACTGATCCCCTCCGTGTTA

60

64

 

Reverse

TCCATCAGGCCTCTATCTGAA

  

Exon 3

Forward

GCTCTCTGTCCCTCACCTTG

63

62

 

Reverse

ACCACCAAGCATTTCCTGAG

  

Exon 4

Forward

CACATTGGAGGTGACAGGAA

63

61

 

Reverse

CCAAGGTGAGACCCAGAAGA

  

Exon 5

Forward

GCTGGCCTTGTTCTATCAGG

62

62

 

Reverse

CTCCCTTCAGTCTCCTGCTG

  

Exon 6

Forward

GGTGAAGCCTGAGTCATTCC

63

62

 

Reverse

CCAACCTACTTCCCCTCCAT

  

Exon 7

Forward

GAGGGATGGAGGGGAAGTAG

61

61

 

Reverse

AGCCAGCACCTAGGGAAAA

  

Exon 8

Forward

GGCACCATTGAAATGCTCTT

59

61

 

Reverse

ATCAAAGTGCCCAGTTACGG

  

Exon 9

Forward

GCAGCTGCTTAACCTGGTAGA

61

61

 

Reverse

ATGTCCACTTGTGCGTGTGT

  

Exon 10

Forward

CCATCCCTGTAAACATGAGG

61

61

 

Reverse

CCCCAATATCTCCACCAGAA

  

Exon 11

Forward

TGGTGGAGATATTGGGGAGA

61

61

 

Reverse

CAAACCCTTCTGACCCTTCA

  

Exon 12

Forward

TGACAGGAGGCGCTAGAGT

62

63

 

Reverse

AGTAGCATAGGCAGGTGGAG

  
 

Forward

CTCCACTCCAGCTTCCTCCT

62

63

 

Reverse

ATCGTTAGCCCCTGTCCTCT

  
 

Forward

TCTCACTTCAGCACATCTTCC

61

63

 

Reverse

GGCCTACCCTGGTCTGTCTT

  
 

Forward

ACCCAAAAGCCCCCTTGT

61

61

 

Reverse

AGTACACTGTGGGGGTGAGG

  
 

Forward

CCAAAGGCACCATTTCTTTT

59

61

 

Reverse

ATGTAGGCGGTGCCTCAG

  

ENaC β

    

Exon 2

Forward

CTAGTCTCCAGGCCCATGAC

62

64

 

Reverse

CTACTGGAAGGGGCTGGAAT

  

Exon 3

Forward

CCCCATGTTCCACACTCTTT

60

62

 

Reverse

AACAAAAATCGATTGCTACCAG

  

Exon 4

Forward

TAATACGGTGCTGCCATTCC

61

63

 

Reverse

GCATAGATCAGCCTGTGTGC

  

Exon 5

Forward

CTCCAGCAGAGCAGGACAAT

62

61

 

Reverse

GGTCTTTCCGCCCTGTGT

  

Exon 6

Forward

GGTGATGGCCTCCCTTCTAT

61

61.5

 

Reverse

AGGCAGCCTGAACACAAGAG

  

Exon 7

Forward

GCTCCATGGGGAGGTACATA

63

63

 

Reverse

GTCCGGCCCTCATAGGTAAG

  

Exon 8

Forward

AAAGTCTCTGGGCTCCAAGG

63

61.5

 

Reverse

AGAGGCCCCCTTGCCTAAC

  

Exon 9

Forward

GAGGGAAGACCCCTGGAAG

62

63.5

 

Reverse

ATACTGGGTGTCGCTGGAAG

  

Exon 10

Forward

TCCTGCAAGTGAGTGTGTCC

62

63

 

Reverse

AGGGGGAGAAGACCCTCTTT

  

Exon 11

Forward

AGCATGTGTGTGCGTGTGTA

60

63

 

Reverse

CAGCAAGAGCAGTTTGGACA

  

Exon 12

Forward

CAACTCAGACTGGAGATGAGCA

62

62.5

 

Reverse

GGAACCATAACCCCCACCTA

  

Exon 13

Forward

CCTAGGTGGGGGTTATGGTT

62

64

 

Reverse

GCAGCCTCAGGGAGTCATAG

  
 

Forward

CTTCCAGCCTGACACAACC

62

63.5

 

Reverse

GCCTGTCTGCTAGGTCAACA

  
 

Forward

CTGAGGGGTTCATAGGGTCA

62

60.5

 

Reverse

ATCCTACACCCCAGACATGC

  

ENaC γ

    

Exon 2

Forward

AGTCGCAGGCTCCAGAGAT

62

64

 

Reverse

GAGGGGCTTTGACATCCAT

  

Exon 3

Forward

GGAAGGCAACATGAAGAAGC

61

60

 

Reverse

ACTGTGAGCCCACCAGCTC

  

Exon 4

Forward

AAGGACCTACCCTGGCATCT

63

60

 

Reverse

CTCAAGGCCTACAGGTGAGC

  

Exon 5

Forward

ACGCAATGCTTCCTGACTTC

60

60

 

Reverse

TGCACTGCTGCTGTCAAGAT

  

Exon 6

Forward

CCCTGGGGACTGCTTTTT

60

60

 

Reverse

TCCATTAGCAGCACCTCCTT

  

Exon 7

Forward

AGGGGAATCCTCCTATCTGG

61

62

 

Reverse

CCTTGGCCTAGATATAGCTTCA

  

Exon 8

Forward

AGGGAGTTCCCGGTCTCTAC

63

61

 

Reverse

GCGTGGCCAAGCTGATTC

  

Exon 9

Forward

AGATGGTGGAGGTTCCACAG

63

60

 

Reverse

GGGAGAAAGGCACAGAGTGA

  

Exon 10

Forward

ACTGGGGCAGGTAGGACTTT

62

60

 

Reverse

GCTTTGGCTGTGTTGCTGTA

  

Exon 11

Forward

CCAAAGCCAGAGACAGGTTG

59

61

 

Reverse

TCGAATGAACGAAAAGGTGA

  

Exon 12

Forward

ACCTGGCAGGAAGCCAAG

62

61

 

Reverse

CCCTCTGGCAGCAAAACTAC

  

Exon 13

Forward

CCCTGAGTGCAGGATTTATCA

61

64

 

Reverse

GTATCTGGGAGGTGGTGTGC

  
 

Forward

GTCAGTGGCACAAAGCCAAG

62

64

 

Reverse

AGCTCATAAGTGCCAAGTCCA

  
 

Forward

CCTGCTGTGAACCGGATA

60

60

 

Reverse

GGCCAACTGTCTGTCTGAGG

  
 

Forward

GCCAGCTATTGCCTGACAT

60

60

 

Reverse

CGCATACTCTCAGTTCAAAGACA

  
 

Forward

GCCAAATGGTATTCCCACAA

59

57

 

Reverse

ATTGGACTAGCCTGGGTGCT