Table 3B Primers designed to screen the 5'flanking sequence of ENaC α, β, and γ genes. Optimum temperatures (°C) for PCR and DHPLC are included in the table.
Sequence | PCR | DHPLC | ||
|---|---|---|---|---|
ENaC α | ||||
Forward | CAGATTCAGCTGCCATGC | 60 | 62 | |
Reverse | AGGCCCTGCAGAACTTGCT | |||
Forward | GGTAGGAAGAGGCAGTGTGC | 61 | 62 | |
Reverse | ACAGGGTTGCAGGAATGTG | |||
Forward | GCTGGTGGTCTCTCCCAGTA | 64 | 62 | |
Reverse | GGTCCCTTCTGATGGAGGTC | |||
Forward | GGGGGACCCATTCTCCTT | 62 | 59 | |
Reverse | GGACTGATGGCGAACTGACT | |||
Forward | CCTTCCTGGCTTTTGTGTGT | 61 | 62 | |
Reverse | GTGTGGCTGGGCTGTGAC | |||
Forward | CAGGCACTGCACCTGTCA | 62 | 62 | |
Reverse | GGCTTAGCGTCTCTCCCTCT | |||
Forward | CACAGAAAGGGAGACCCAAA | 60 | 63 | |
Reverse | GGTCTTGCTCCTTGAATTGG | |||
Forward | GAGGGCAGCCTGGGATGCGG | 61 | 62 | |
Reverse | TTATAATAGCAATAGCCCCA | |||
Forward | TCCAAGGAGAAGGCGCCCCCA | 61 | 62 | |
Reverse | AGGGCTGGGTGAGAGGAT | |||
Forward | GGTTTAAGGATTTGCTTGATTC | 61 | 61 | |
Reverse | TGTTCTGCAAGGACAGCATC | |||
Forward | CAAAGTACCCAATATCTATT | 61 | 61 | |
Reverse | AGGGCTGGGTGAGAGGAT | |||
Forward | CCTGGTTTTGGGGTGTGT | 61 | 61 | |
Reverse | TGTTCTGCAAGGACAGCATC | |||
Forward | GCTCTCTTTGGGCTGTGGGGAC | 61 | 61 | |
Reverse | TGTTCTGCAAGGACAGCATC | |||
ENaC β | ||||
Forward | GGTCTTCTGGGAAGAGCAAA | 61 | 62 | |
Reverse | CACGGTTCCATTTCCTGTGT | |||
Forward | CAGCCATGCACATGAAGACT | 61 | 57 | |
Reverse | TTGCAGCCAACTTGACTAGAT | |||
Forward | TGGTGATACCCTTCTTGTTGG | 61 | 60 | |
Reverse | CCTCCGTGTATCCTCACTCC | |||
Forward | AAAATAGAACGGGGCTGTGA | 61 | 62 | |
Reverse | GGAGCTGGGTCACCTGTAGA | |||
Forward | TGACCTGGAGCCTGTCAACT | 62 | 64 | |
Reverse | GACGGAACTGCGGTCATT | |||
ENaC γ | ||||
Forward | CTTGACATGTTTCTACCCACCA | 61 | 58 | |
Reverse | TTAGAACGCTGAAACCGTGA | |||
Forward | CATCCTCAACTCAAGAATCTGC | 60 | 60 | |
Reverse | CCACTCTGCAAGCTGCATTA | |||
Forward | TGCAGAAGCAGCAGTAAGAGA | 61 | 62 | |
Reverse | CTGGCGTGTGTACAGTCTGG | |||
Forward | CTTCTGGCCGTGCCACTT | 61 | 62 | |
Reverse | TCGGGCTCCTCTTTCAACTA | |||
Forward | CGCGGGAAAGCTTTTCTTTA | 61 | 63 | |
Reverse | CTTGCTCCCTTCCCTCTCAC | |||
Forward | ATGAACAGAGCCTCGCAAAC | 61 | 62 | |
Reverse | GATAGTGGTCGCAGGTGACA | |||
Forward | GGTGGAAACGTTGGAATAGG | 62 | 58 | |
Reverse | AAAGCAAGGCTGTCGCTCTA |