Pedigree (A) and electropherograms (B) showing a mutation in the bovine LRP4 gene detected in Holstein family II. (A) Pedigree chart of the Holstein family II used in this study. Animals affected with syndactyly are indicated as solid black symbols. Samples from animals indicated by arrows were available for the molecular genetic analyses. Phenotypic data from the other animals were obtained from the farmers records. (B) Sequence analysis of genomic DNA of the investigated animals. An arrow denotes the position of the point mutation. Note that the affected animal (V-1) is homozygous for the c.4940C>T mutation while its parents (IV-1 and IV-2) are heterozygous carriers of this mutation. Numbering of nucleotides and codons is according to the open reading frame of the cDNA sequence as deposited in GenBank (GenBank accession no. DQ462703).