Pedigree (A) and phenotype (B) and electropherograms (C) showing a mutation in the bovine LRP4 gene detected in Simmental family. (A) Pedigree chart of the Simmental family used in this study. Animals affected with syndactyly are indicated as solid black symbols. Samples from animals indicated by arrows were available for the molecular genetic analyses. Phenotypic data from the other animals were obtained from the owners records. (B) German Simmental calf (IV-1) affected with syndactyly. Forefeet (left) and hindfeet (right). (C) Sequence analysis of genomic DNA of the investigated animals. An arrow denotes the position of the point mutation. Note that the affected animal (IV-1) is homozygous for the c.241G>A and the c.3595G>A mutations, respectively, while its parents (III-1 and III-2) show the heterozygous genotypes. Numbering of nucleotides and codons is according to the open reading frame of the cDNA sequence as deposited in GenBank (GenBank accession no. DQ462703).