Pedigree (A) and phenotype (B) and electropherograms (C) showing a mutation in the bovine LRP4 gene detected in crossbred family. (A) Pedigree chart of the Crossbred family used in this study. Animals affected with syndactyly are shown as solid symbols. Samples from animals indicated by arrows were available for the molecular genetic analyses. Phenotypic data from the other animals were obtained from the owners records. (B) Adult bull (II-1) with syndactyly. Forefeet (left) and hindfeet (right). (C) Genotypes at c.2719G>A and c.4863_4864delCGinsAT for the six available animals. Note that both affected animals are heterozygous for both mutations. (D) Sequence analysis of genomic DNA of affected and a unrelated wildtype control animals. An arrow denotes the position of the mutations. Numbering of nucleotides and codons is according to the open reading frame of the cDNA sequence as deposited in GenBank (GenBank accession no. DQ462703).