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Figure 2 | BMC Genetics

Figure 2

From: Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

Figure 2

Agarose gel electrophoresis of PCR amplified fragments of the area surrounding the tbp and tfb genes of Halobacterium sp. NRC-1. Template DNA used in Lane 1 of each set is genomic DNA from a population of deletant candidates (roughly 1000 colonies grown on CM+-5FOA agar). Template DNA used in Lane 2 is genomic DNA from the parental strain. Lanes with a higher and lower band are representing the wild-type and knockout allele, showing the transcription factor is non-essential. For the tbp genes the knockout allele is roughly 500 bp below the wild-type allele for the tfb genes it is roughly 900 bp below.

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