Standard curves of the Quantitative real-time PCR. Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into pMD18-T vectors. The log ranges of the standard curves are from 106 to 102 copies. Threshold cycles of each standard dilution point were used to generate the standard curve for each gene. Linear equations corresponding to each standard curve are given, and R2 for each standard curve is more than 0.99.