Polymorphisms of Glutathione S-transferases Omega-1 among ethnic populations in China
© Fu et al; licensee BioMed Central Ltd. 2008
Received: 12 December 2007
Accepted: 10 April 2008
Published: 10 April 2008
Glutathione S-transferases (GSTs) is a genetic factor for many diseases and exhibits great diversities among various populations. We assessed association of the genotypes of Glutathione S-transferases Omega-1 (GSTO1) A140D with ethnicity in China.
Peripheral blood samples were obtained from 1314 individuals from 14 ethnic groups. Polymorphisms of GSTO1 A140D were measured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Logistic regression was employed to adjustment for regional factor. The frequency of GSTO1 140A allele was 15.49% in the total 14 ethnic populations. Compared to Han ethnic group, two ethnic populations were more likely to have AA or CA genotype [odds ratio (OR): 1.77, 95% confidence interval (95% CI): 1.05–2.98 for Uygur and OR: 1.78, 95% CI: 1.18–2.69 for Hui]. However, there were no statistically significant differences across 14 ethnic groups when region factor was adjusted. In Han ethnicity, region was significantly associated with AA or CA genotype. Han individuals who resided in North-west of China were more likely to have these genotypes than those in South of China (OR: 1.63, 95% CI: 1.21–2.20).
The prevalence of the GSTO1 140A varied significantly among different regional populations in China, which showed that geography played a more important role in the population differentiation for this allele than the ethnicity/race.
Glutathione S-transferases (GSTs) constitute multifunctional enzymes that metabolize the biotransformation and disposition of a wide range of exogenous and endogenous compounds via glutathione (GSH) conjugation . Seven sub-classes of GSTs have been identified in humans, including Alpha, Mu, Pi, Theta, Zeta, Sigma and Omega . The Omega GSTs (GSTO), a new subfamily of the human GSTs was identified through analysis of the expressed sequence tag (EST) database and sequence alignments [2, 3]. They have a cysteine residue in the active site compared to a tyrosine or serine residue in other mammalian GSTs . A type of the Omega GSTs, GSTO1, has a special activity of reducing monomethylarsonic acid (MMAV) to monomethylarsonous acid (MMAIII), which appears to be the rate-limiting step in the biotransformation of inorganic arsenic . As a notorious environmental carcinogen, inorganic arsenic is responsible for endemic arsenicosis because of arsenic contamination of drinking water in many regions of the world. Chronic exposures can lead to hyperkeratosis and loss of skin pigmentation as well as cancers of the skin, bladder, and lung.
Variability in human arsenic metabolism could be an underlying determinant of individual susceptibility to arsenic-induced disease in humans. A140D and T217N of GSTO1 variants reduce enzyme activity and the inhibited inorganic arsenic biotransforming capacity . Some literatures reported that GSTO1 A140D substitution had a relationship with cancer, vascular dementia and stroke [7, 8]. In several previous researches, the frequency of GSTO1 A allele at position 140 is different in some regions and races, such as Brazilian (f = 0.156) , Australian (f = 0.335), Japanese (f = 0.118), Chinese (f = 0.165) , German (f = 0.32) , African (f = 0.081), EA (European ancestry American) (f = 0.30–0.35), AA (African ancestry American) (f = 0) . In this study, we analyzed distribution of the GSTO1 A140D polymorphisms among 14 ethnic populations in China.
Because the change of the GSTO1 allele C to allele A at position 140 removes one of the two Cac8I restriction sites in the amplified fragment, the GSTO1 140 A/A yielded 265 bp, 145 bp and 61 bp fragments, the GSTO1 140A/D yielded 410 bp, 61 bp, 265 bp and 145 bp fragments, whereas the GSTO1 140 D/D yielded 410 bp and 61 bp fragments.
Distribution of the GSTO1 140 mutant allele frequency in the 14 Chinese ethnic populations
Mutant allele A (10-4)
Association of ethnicity and genotype CA or AA in China
Unadjusted Odds Ratio
Region adjusted Odds Ratio
95% confidence interval
Association of region with genotype CA or AA among Han ethnic individuals
95% confidence interval
We assessed ethnic variation of the GSTO1 A140D mutant allelic frequency among 14 Chinese ethnic populations. The genotypic distribution varied across regions, which support the previous study. It has been reported that genetic structure, particularly the Y chromosome and mitochondrion study differed by regions [12, 13]. As different groups belong to different regions, the variances of genetic structure between different groups lay on their large geographical span.
It is demonstrated that the observed ethnic variation was not significant once geographic region was accounted for. We further evidenced that region was associated with the genotype. These findings support that genotype of CA and AA distribution is likely to be determined by environmental factors rather than ethnicity.
Our study further demonstrated the regional difference appeared the comparison between North-west and South of China. The result suggests that environmental factors play a significant role in the formation of genetic characteristics in Chinese populations. Migration between north-west and the south of China might be rare in ancient time because of lack of transportations to overcome numerous huge mountains and other barriers. That might inhabit gene flow across regions. On the other hand, most of the northern areas are plains. This geographic character, as well as the long history of wars and famines in the north, may have contributed to plentiful gene flows among the northern populations. Populations from northwest of China showed comparatively higher mutation allele frequency, probably due to the ancestral gene flow from Europe and recent intermarriage amongst the northwest groups.
By further literature review of the allelic frequencies in the world, we found the prevalence of the allele varied substantially between continental populations. For example, the frequency of GSTO1 140A is 0.32 in German,  0.30–0.35 in European ancestry American , 0.118 in Japanese and 0.165 in Chinese , but only 0.081 in African population and even absent in African ancestry American . The present results of variation of the SNP frequencies with geographical regions are mainly attributable to the different population historical events of the geographically separated population groupings.
This study has several limitations. Firstly, besides A140D substitution, there are several other common polymorphisms that can induce change of amino acids in GSTO1 exons. In those polymorphic sites, E208K and E155del exist polymorphisms in the Chinese  and both mutant frequencies are very low. We could not obtain the accurate data on allele distribution of these two polymorphisms in our samples. Secondly, we only included 14 ethnic populations out of 56 ethnic populations. However, it is less likely to observe significant ethnic variation even if more ethnic groups were recruited. Thirdly, we also studied T217N and A236V sites in relatively large ethnic populations of Hui, Mongol, Sichuan Han and Hazakh. The additional analysis did not find any polymorphism. Therefore, it is necessary to enlarge sample sizes for other ethnic samples.
The prevalence of the GSTO1 140A varied significantly among different regional populations in China, supporting that geography played a more important role in the population differentiation than ethnicity or race. To some degree, our study findings suggest that ethnicity not be considered in the study of causal relationship between genetic polymorphisms and disease.
Geographic location of study population
Longitude and latitude
Minqing County, Fujian Province,
Chengdu City, Sichuan Province,
Yining, Xinjiang Autonomous Region
Arlihe, Inner Mongolia Autonomous Region
Molidawa, Inner Mongolia Autonomous Region
Yanji City, Jilin Province
Molidawa, Inner Mongolia Autonomous Region
Tongjiang, Heilongjiang Province
Xiuyan City, Liaoning Province
Wuqia, Xinjiang Autonomous Region
Yining City, Xinjiang Autonomous Region
Hailar City, Inner Mongolia Autonomous Region
Tongxin County, Ningxia Autonomous Region
Yili, Xinjiang Autonomous Region
Yining City, Xinjiang Autonomous Region
Guizhou City, Yunnan Province
Genomic DNA was extracted from peripheral blood anti-coagulated with ACD by a standard phenol-chloroform protocol. GSTO1 140 variants were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR was performed in 20 ul volumes of a mixture containing 50 ng DNA template, 0.2 uM each primer, 200 uM dNTPs, 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl (pH 8.3) and 2.0 U Taq DNA polymerase (Takara). The forward and reverse primers are 5'-aacgtgggtacaatttcc-3' and 5'-ccaggactgtaagggttc-3', respectively. Amplification was carried out for 32 cycles at 94°C 30 s, 55°C 30 s and 72°C 30 s with Eppendorf AG. Then the PCR products were digested with Cac8I restriction endonuclease (5'-GCN/NGC-3') at 37°C for 14 h, and the digested products were detected on 2.5% agarose gels.
Deviations from Hardy-Weinberg equilibrium (HWE) were investigated for this locus using the chi-square statistic, with expected frequencies derived from allele frequencies. We calculated the allele and genotype frequencies by direct counting and tested the significant differences among the populations using the chi-square. Logistic regression was fitted using genotype 'AA or CA' as dependent variable, and ethnicity and regions as independent variables to test variation of genotype across ethnicity after adjustment for regions. Data were analyzed using SAS software (version 8.2; SAS Institute Inc., Cary, NC, USA).
The National Natural Science Foundation of China (No. 30530640) funded this study. We also gratefully acknowledge the numerous sample donors for making this work possible.
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