Table 3 Primers and probe employed in these experiments
Function | Primer name | Primer and probe sequences (5'-3') | Tm |
|---|---|---|---|
Cloning | GAPF | CTCCAAAGTCACACGCTCAGAAC | 62 |
GAPR | ATAGTCCTACCCCACGAAGAAGC | ||
5GSP | GGAAGGGTATGCAGGAGCTGAAGTAGGC | 71 | |
Subcellular | P4GFPF | CGGCTCGAGGGATGGGGGCTAACC | 63 |
localization | P4GFPR | GAGAAGCTTAGAGTTTGGTGTGGCG | |
Real-time | Real-time F | CCCAGGAAATGCTTCTGAACTAG | 60 |
PCR | Real-time R | CCCACAAAGAATCTCTGATAAAGC | |
ProbeP4 | AAGCTGTTGTTTTGGGGAGCACTG | ||
ACTB F | GGATGCAGAAGGAGATCACG | 60 | |
ACTB R | CTCGTCGTACTCCTGCTTGC | ||
ProbeBeta | ATCAAGATCATCGCGCCTCCCGAGC | ||
Mapping | MapF | TGGCAGAGCGGTCGTCACTTAGGC | 65 |
MapR | GAGCAGAATCTCCTCCCACGCACCAT | ||
SNPs detection | SNPF1 | CTAGAACCACTCAAACCAAGCAGC | 62 |
SNPR1 | ATCAGGCAGGTAAAAGGATAACGG | ||
SNPF2 | GCCTTCTGAGTAGCAGTATGAGTTG | 62 | |
SNPR2 | CCTGCGAGAACTGAGAATAATCC |