Growth of tra1
double mutant strains in sorbitol. Strains containing the indicated single disruptions (mdm34, swc3, cog5 and mon2) and combinations with tra1SRR 3413(tra1) were generated by sporulation of diploids obtained from SGA analysis. These strains plus CY2222 (tra1SRR 3413) and BY7092 (TRA1) were grown overnight to saturation in YPD at 30°C. Cells were diluted to approximately 2000 cells per μl and 5 μl of 10-fold serial dilutions spotted on synthetic complete media containing 2% glucose without (SC) or with 1.0 M sorbitol. Cells were grown for 3 days (upper grouping) or 2 days (lower grouping) at 33.5°C.