Phenotypic comparison of tra1
with deletions of SAGA/SLIK and NuA4 components. Yeast strains BY3534 (ngg1Δ0), BY4741 (wild-type background for deletion strains), BY7285 (gcn5Δ0), BY4282 (adaΔ0), BY3281 (spt7Δ0), BY4240 (yaf9Δ0), BY2940 (eaf7Δ0), BY7092 (TRA1) and CY2222 (tra1SRR 3413) were grown overnight to saturation in YPD at 30°C. Cells were diluted to approximately 2000 cells per μl and 5 μl of 10-fold serial dilutions spotted on synthetic complete media containing 2% glucose (SC), with 2 μg/ml staurosporine (ST), 7.5 μg/ml calcofluor white (CW), or 2 μg/ml staurosporine plus 7.5 μg/ml calcofluor white (CW ST) or YPD with 2 nM rapamycin (RAP). SC and ST were grown for 2 days at 30°C, CW and CW ST for 3 days, and RAP for 4 days. For the rapamycin image, TRA1 and tra1
were grown on a separate plate.