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Table 1 Immune responses (mean ± SD) and analyses of variance in the group immunized with KLH

From: Correlated effects of selection for immunity in White Leghorn chicken lines on natural antibodies and specific antibody responses to KLH and M. butyricum

  

Line

Measure

Sample size

ND3-L

PHA-L

CC-L

Control

Trait under selection

   ND3

48

5.75 ± 1.65a

3.00 ± 1.54b

1.64 ± 1.06c

2.56 ± 1.34b

   PHA

48

1.13 ± 0.44c

1.97 ± 0.92a

1.34 ± 0.73c

1.68 ± 0.81b

   CC

48

  

0.30 ± 0.07a

0.17 ± 0.05b

Before immunization with KLH (d 0)

   KLH

50

3.89 ± 1.45

4.00 ± 1.22

3.78 ± 1.23

3.69 ± 1.09

   LPS

50

1.95 ± 1.73

1.85 ± 1.58

2.27 ± 1.93

1.99 ± 1.64

   LTA

50

4.90 ± 1.51a

4.84 ± 1.43a

4.08 ± 1.32b

3.93 ± 1.23b

   Anti-Gal

50

13.68 ± 3.82a

12.88 ± 3.03a

10.89 ± 2.92b

10.75 ± 2.46b

After immunization with KLH (d 7)

   KLH

50

8.95 ± 1.95

9.23 ± 2.24

8.04 ± 2.22

8.85 ± 2.06

   LPS

49

4.49 ± 1.85ab

4.08 ± 1.61bc

3.40 ± 2.15c

5.02 ± 2.32a

   LTA

48

6.12 ± 1.21a

5.93 ± 1.20a

5.17 ± 1.33b

5.44 ± 1.18b

   Anti-Gal

50

20.08 ± 5.22

18.97 ± 4.15

19.64 ± 3.82

18.50 ± 3.02

  

Analysis of variance

  

Line

Sex

Interaction

R 2

Trait under selection

   ND3

48

***

ns

ns

0.79

   PHA

48

***

ns

ns

0.72

   CC

48

***

ns

ns

0.83

Before immunization with KLH (d 0)

   KLH

50

ns

ns

*

0.55

   LPS

50

ns

ns

ns

0.53

   LTA

50

***

ns

ns

0.64

   Anti-Gal

50

**

ns

ns

0.76

After immunization with KLH (d 7)

   KLH

50

ns

ns

ns

0.71

   LPS

49

*

*

ns

0.71

   LTA

48

***

ns

ns

0.65

   Anti-Gal

50

ns

**

ns

0.76

Responsiveness (d 7-d 0)

   KLH

50

ns

ns

ns

0.72

   LPS

50

***

ns

ns

0.75

   LTA

50

ns

ns

ns

0.73

   Anti-Gal

50

ns

***

ns

0.75

  1. a-c: Means in the same row with no common superscript differed significantly (P ≤ 0.05) based on Duncan's multiple range test.
  2. 1 Antibody titers for to KLH, LPS and LTA are the log2 of the reciprocal of the antibody dilution.
  3. 2Antibody titers to anti-Gal epitope measured in plasma samples as the agglutination titer of glutaraldehyde stabilized rabbit. Titers are expressed as the double of the column number of the last plasma dilution showing clear evidence of agglutination. Responsiveness, measured as difference between the Ab titers at day 0 and day 7 post KLH immunization
  4. ND3-L = line selected for high antibody response to ND3 3 wk after vaccination.
  5. PHA-L = line selected for high cell-mediated immune response, to PHA at 9 wk of age.
  6. CC-L = line selected for high phagocytic activity, measured as carbon clearance at 12 wk of age.
  7. Control = line selected at random
  8. * P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.