Table 2 Primer sequences.
Name | Position | Direction | Sequence 5'-3' |
|---|---|---|---|
ML5'-1 | 5'-UTR | Forward | GACTGCCCGTGGCCCCATTATTTG |
ML1F | 5'-UTR | Forward | GCTTTAAGACCGGTTGGATCTAC |
SJ93 | Exon 1 | Forward | GGGGAGGAAGCTGGATCTCTC |
RACE4F | Exon1 | Forward | ATGGGGAGGAAGCTGGATCTCTCCAAG |
RACE2R | Exon 1 | Reverse | ACCTCCCAGACATGCTTGGCCTCCTC |
RFLP-F1 | Intron 1 | Reverse | ACACTGACACAGCTGATAACATCAC |
MV2R | Exon 2 | Forward | GAAGTGCAAGATAGACCAGGAAAG |
RACE1R | Exon 2 | Reverse | GCTTGCTGTTCAGCAGGAACTTGAAGG |
MV18F | Exon 3 | Forward | GAAGGGGAATTCAGCTCTTCTAGG |
ML14F | Exon 5–6 | Forward | CAGAGTGCTACAGCAGAATGC |
BlotE91F | Exon 8–9 | Forward | CTCGGGAAGTCAGATTCATGAGC |
ML3R | Exon 10 | Reverse | CTTTCTCTTCAACTCCTCCTCCTC |
ML16F | Exon 12 | Forward | GTCTACCTGACTGCTGGGAAGG |
RACE5F | Exon 15 | Forward | GCCACAGGTCCTCAGGAGGAGGTTC |
MV8R | Exon 16 | Forward | ATGACTCCTTTGACCGCAACTC |
MV6F | Exon 17 | Reverse | AGGACAGGTGGGTCATCACTG |
ML15R | Exon 17 | Reverse | CTCAGGACAGGTGGGTCATCACTG |
ML3'-1 | 3'-UTR | Reverse | GATGAGGCAGGGTGGCAGGACCAG |