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Figure 1 | BMC Genetics

Figure 1

From: Differentially methylated CpG island within human XIST mediates alternative P2 transcription and YY1 binding

Figure 1

Xi-specific DNase I hypersensitive site ~1.5 kb within XIST. A) Schematic indicating DHS sites from ENCODE genome-wide survey with locations of primers used for qPCR-based DHS assay. Shading of bars reflects increasing amounts (0, 10, 20 or 40 U) of DNase. B) qPCR-based DHS assay on female and male lymphoblasts, Xi and Xa mouse-human hybrids and an HT1080 (male fibrosarcoma) cell line containing a DOX-inducible XIST transgene (HT1080XISTi). Sensitivity of biological triplicates ± standard deviation is shown relative to an unsensitive region. JPX is used as a positive control. *p = 0.05-0.01, **p = 0.01-0.001, ***p < 0.001, n.s. = not significant.

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BMC Genomic Data

ISSN: 2730-6844

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