Figure 2

Xi-specific DHS site correlates with an actively transcribing P2 promoter. A) Schematic of XIST exon 1 indicating the locations of primers used for qRT-PCR analysis. The outlined boxes are UCSC tracks showing chromatin state segmentation as determined by compiled ChIP-seq data (upper) and RNA-seq data for the region (lower) [38],[39]. B) Sequenced 5`RACE products with arrows indicating transcription orientation. C) Levels of XIST upstream and downstream of P2 determined by qRT-PCR (shown as biological triplicates ± standard deviation). D) Strand-specific qRT-PCR of P2as transcripts relative to sense XIST (average of biological triplicates ± standard deviation).