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Figure 3 | BMC Genetics

Figure 3

From: Transcriptional regulation of the human, porcine and bovine OCTN2 gene by PPARα via a conserved PPRE located in intron 1

Figure 3

Activation of porcine, bovine and human OCTN2-PPRE reporter gene constructs by PPARα. HepG2 cells were transfected with either (A) wild-type plasmid pGL4.23-pOCTN2-PPRE-Luc or mutant plasmid pGL4.23-pOCTN2-PPREmut-Luc or (B) wild-type plasmid pGL4.23-bOCTN2-PPRE-Luc or mutant plasmid pGL4.23-bOCTN2-PPREmut-Luc or (C) wild-type plasmid pGL4.23-hOCTN2-PPRE-Luc or mutant plasmid pGL4.23-hOCTN2-PPREmut-Luc. Cells were co-transfected with pCMX-mPPARα and pCMX-mRXRα or pCMX (empty vector) expression plasmids, and pGL4.74-RLuc as internal control and normalization plasmid. The plasmids pGL4.23-luc and 3xACO-PPRE were used as negative and positive control plasmids, respectively. 12 h after transfection cells were cultured in medium in the presence or absence of 50 μM WY-14,643 for 24 h and, subsequently, luciferase activities determined by luminometry. Bars represent means ± SD for one out of three independent experiments each performed in triplicate. The other experiments revealed similar results. *Different from control (empty vector pCMX), P < 0.05.

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