Expression of BoFAD3 genes in wt and low α-linolenic acid (C18:3) mutant B. olereacea var. alboglabra lines. (A) Semi-quantitative RT-PCR of BoFAD3-1 and BoFAD3-2 expression in developing siliques (25-30 days after pollination, DAP) from wt and two low C18:3 mutant lines. BoPP2AA3 expression was analyzed as an internal control. (B) Quantitative real-time RT-PCR analysis of BoFAD3-2 using DNA-free total RNA derived from developing siliques (25-30 DAP) from two wt and low C18:3 mutant lines, respectively. Each block represents the relative mean level of BoFAD3-2 transcript normalized to the constitutively expressed internal control, BoGAPDH, from two biological and three technical replicates. Bars denote standard deviations. NTC, no template control; Ladder, molecular weight ladder.