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Figure 6 | BMC Genetics

Figure 6

From: Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a ‘class a’ FATTY ACID DESATURASE 3gene

Figure 6

Activity of wt and mutant BoFAD3-2 proteins in Saccharomyces cerevisiae . FAMEs derived from three independent colonies expressing either wt BoFAD3-2, mutant BoFAD3-2, or empty vector grown under inducing conditions and supplemented with 150 μM linoleic acid (C18:2) were analyzed by GC-MS to determine desaturase activity in each case. Blocks indicate mean percent conversion from C18:2 to α-linolenic acid (C18:3) of three biological and two technical replicates. Bars represent standard deviations.

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