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Table 2 Details of the primers used

From: Genetic diversity analysis in a set of Caricaceae accessions using resistance gene analogues

Marker name

Gene

Protein

Ann temp

Exon no.

Expected size of amplification product in rice in bp

Forward primer

Reverse primer

BDTG 1

Xa1

P LOOP

59.8

1

508

5′ -ATTAATCCACGACGACCAGG – 3′

5′ -GTAGCACAAGCACCTCCTCC – 3′

BDTG 2

KINASE 2

60

2

429

5′ -GAGGAGGTGCTTGTGCTACAG – 3′

5′ -GGCACTGGCATTACCTTGAT – 3′

BDTG 3

TRANS MEM

59.5

3

519

5′ -GGTGAGGGTGCATCAAATG – 3′

5′ -TTATTCCTTCGTGGCTCTGG – 3′

BDTG 4

59.8

3

531

5′ -TTGGATCATGTCTCCAACCA – 3′

5′ -ACTTCAGCGCTTGCATGAT – 3′

BDTG 5

59.8

3

877

5′ -CATCTATCCAACCCCTTACAGC – 3′

5′-CAAGCTTGTTCATGGATTTCAA – 3′

BDTG 6

60.2

3

1778

5′ -TAGAACTCAGGAGGAGGCATGT – 3′

5′ -TGATTGCGGAAGGATACACA – 3′

BDTG 7

60.2

3

570

5′ -AGATGGAATGTGTATCCTTCCG – 3′

5′ -GGAAGGATACACCTTCCATTTTC – 3′

BDTG 8

LRR

59.5

4

719

5′ -GATGGCTCCTACCGCTATCA – 3′

5′ -GATGTGCAAGAATGGAGCTG – 3′

BDTG 9

60.9

4

569

5′ -CTCAAATTTAGTGTCTCTGCAGCTC – 3′

5′ -TCCGCGATAGTTAAGCTCTAGG – 3′

BDTG 10

60

4

735

5′ -TCTGCAAGCACCTCACCTC –3′

5′ -ATGCATTGGAGCGGATTG – 3′

BDTG 11

xa5

TF II A

59.9

1

258

5′ -TTCGAGCTCTACCGGAGGT – 3′

5′ -AGAAACCTTGCTCTTGACTTGG – 3′

BDTG 12

60.2

2

141

5′ -TGTTCTTTTCTCAGGGCCAC – 3′

5′ -AGTTTGGAATCACAGGCCAC – 3′

BDTG 13

Xa26

RECP KINASE

59.5

1

594

5′ -GATGCATACTCTTGCTGCCA – 3′

5′ -CAAGACTGTGCAACCCCTG – 3′

BDTG 14

60.1

1

652

5′ -ACCAGCTATACGGTCCAATCC – 3′

5′ -GCAAGATGCAACCATGAAAGT – 3′

BDTG 15

59.6

1

616

5′ -CTATTCCTGCTTCTCTTGGCA – 3′

5′ -AGCCTGACGATTTTATCAAGATG – 3′

BDTG 16

59.6

1

636

5′ -CATCTTGATAAAATCGTCAGGCT – 3′

5′ -GGTTGCACGAAGAAGCTCAT – 3′

BDTG 17

59.8

1

524

5′ -CGATGATAGCATGTTGGGC – 3′

5′ -AAAAACTATTAAGTACCTGGTGCCAT– 3′

BDTG 18

59.9

1

567

5′ -TGAGCAGAGTATGGGACTCTAGG – 3′

5′ -ACACCAACTATAAATTGTTGCAGAAC – 3′

BDTG 19

Xa27

59.9

1

391

5′ -GAAGCCACACACACTGAGACA – 3′

5′ -CGGAGGAGAACTAGAGAGACCA –3′

BDTG 20

Xa21

SIGNAL

59.7

1

200

5′ -CACTCCCATTATTGCTCTTCG – 3′

5′ -ACACAACACCCACCCATGT – 3′

BDTG 21

LRR

61.8

2

500

5′ -GCTCCTCCAACCTGTCCG – 3′

5′ -TAAACGCTCTTAGAGACGAAAGGT – 3′

BDTG 22

59.7

2

591

5′ -CAATTCTATCTGGAACCTTTCGTC – 3′

5′-ACCGCTCAAGTTGTTTTCGT – 3′

BDTG 23

60

2

601

5′ -GGCATTCTACTCGCCTACGA – 3′

5′ -GCATTGCCTTGGATTGAGAT – 3′

BDTG 24

CHARGED

59.8

3

707

5′-TGCCTCGATGTTGTCCATTA – 3′

5′ -TCAATGAGGTCCCATCAACA – 3′

BDTG 25

KINASE

60.1

4 & 5

1268

5′ -AGGGACAATTGGCTATGCAG – 3′

5′ -AGAATTCAAGGCTCCCACCT – 3′

BDTG 26

Xa21(A1)

LRR

59.8

1

280

5′ -TGTTGTTCTCTGCGCTGC – 3′

5′ -CGTCCTGAGGAAGGATAGGTT –3′

BDTG 27

59.6

1

408

5′ -CATCGCTGGGCAACCTAT – 3′

5′ -TTGGACACGACTTCAAATATGG – 3′

BDTG 28

59.6

1

397

5′-CCCAGATCCTATTTGGAACATC – 3′

5′ -TGGAAACAGAATCAGGGAGG – 3′

BDTG 29

59.9

1

410

5′ -AGGTTGCAAATTTGGTGGAG – 3′

5′ -GGAATGCTAAATATTTCAATGGGA – 3′

BDTG 30

60.2

1

391

5′ -TAGGGCAAATTCCCATTGAA – 3′

5′ -AAAACACCATTGGTTGGCA – 3′

BDTG 31

59.9

1

405

5′ -CTTTCGTTCAACAGCTTCCAC – 3′

5′ -CACCATCTTGACTATCAAATTCTCC – 3′

BDTG 32

59.9

1

563

5′ -CTTTCGTTCAACAGCTTCCAC – 3′

5′ -CAATGAAAGGAGGTAGACATAAACAGT – 3′

BDTG 33

SNAP

60.2

2

215

5′ -ACTGTTTATGTCTACCTCCTTTCATTG – 3′

5′ -AATAGATTTGCTACGGTCGAACA – 3′

BDTG 34

KINASE

59.7

3

363

5′ -TTTGTTATGGAATTCTAGTGTTGGAA – 3′

5′ -CCAACATAACATCAGCATGTCTC – 3′

  1. Gene - Resistance genes from which they were designed; Protein - Protein coded by the DNA sequence amplified by the corresponding marker; Ann Temp – Annealing Temperature of the respective primer pair; Exon no. - Exon of the original gene from which primer pair was designed.