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Table 2 Details of the primers used

From: Genetic diversity analysis in a set of Caricaceae accessions using resistance gene analogues

Marker name Gene Protein Ann temp Exon no. Expected size of amplification product in rice in bp Forward primer Reverse primer
BDTG 1 Xa1 P LOOP 59.8 1 508 5′ -ATTAATCCACGACGACCAGG – 3′ 5′ -GTAGCACAAGCACCTCCTCC – 3′
BDTG 2 KINASE 2 60 2 429 5′ -GAGGAGGTGCTTGTGCTACAG – 3′ 5′ -GGCACTGGCATTACCTTGAT – 3′
BDTG 3 TRANS MEM 59.5 3 519 5′ -GGTGAGGGTGCATCAAATG – 3′ 5′ -TTATTCCTTCGTGGCTCTGG – 3′
BDTG 4 59.8 3 531 5′ -TTGGATCATGTCTCCAACCA – 3′ 5′ -ACTTCAGCGCTTGCATGAT – 3′
BDTG 5 59.8 3 877 5′ -CATCTATCCAACCCCTTACAGC – 3′ 5′-CAAGCTTGTTCATGGATTTCAA – 3′
BDTG 6 60.2 3 1778 5′ -TAGAACTCAGGAGGAGGCATGT – 3′ 5′ -TGATTGCGGAAGGATACACA – 3′
BDTG 7 60.2 3 570 5′ -AGATGGAATGTGTATCCTTCCG – 3′ 5′ -GGAAGGATACACCTTCCATTTTC – 3′
BDTG 8 LRR 59.5 4 719 5′ -GATGGCTCCTACCGCTATCA – 3′ 5′ -GATGTGCAAGAATGGAGCTG – 3′
BDTG 9 60.9 4 569 5′ -CTCAAATTTAGTGTCTCTGCAGCTC – 3′ 5′ -TCCGCGATAGTTAAGCTCTAGG – 3′
BDTG 10 60 4 735 5′ -TCTGCAAGCACCTCACCTC –3′ 5′ -ATGCATTGGAGCGGATTG – 3′
BDTG 11 xa5 TF II A 59.9 1 258 5′ -TTCGAGCTCTACCGGAGGT – 3′ 5′ -AGAAACCTTGCTCTTGACTTGG – 3′
BDTG 12 60.2 2 141 5′ -TGTTCTTTTCTCAGGGCCAC – 3′ 5′ -AGTTTGGAATCACAGGCCAC – 3′
BDTG 13 Xa26 RECP KINASE 59.5 1 594 5′ -GATGCATACTCTTGCTGCCA – 3′ 5′ -CAAGACTGTGCAACCCCTG – 3′
BDTG 14 60.1 1 652 5′ -ACCAGCTATACGGTCCAATCC – 3′ 5′ -GCAAGATGCAACCATGAAAGT – 3′
BDTG 15 59.6 1 616 5′ -CTATTCCTGCTTCTCTTGGCA – 3′ 5′ -AGCCTGACGATTTTATCAAGATG – 3′
BDTG 16 59.6 1 636 5′ -CATCTTGATAAAATCGTCAGGCT – 3′ 5′ -GGTTGCACGAAGAAGCTCAT – 3′
BDTG 17 59.8 1 524 5′ -CGATGATAGCATGTTGGGC – 3′ 5′ -AAAAACTATTAAGTACCTGGTGCCAT– 3′
BDTG 18 59.9 1 567 5′ -TGAGCAGAGTATGGGACTCTAGG – 3′ 5′ -ACACCAACTATAAATTGTTGCAGAAC – 3′
BDTG 19 Xa27 59.9 1 391 5′ -GAAGCCACACACACTGAGACA – 3′ 5′ -CGGAGGAGAACTAGAGAGACCA –3′
BDTG 20 Xa21 SIGNAL 59.7 1 200 5′ -CACTCCCATTATTGCTCTTCG – 3′ 5′ -ACACAACACCCACCCATGT – 3′
BDTG 21 LRR 61.8 2 500 5′ -GCTCCTCCAACCTGTCCG – 3′ 5′ -TAAACGCTCTTAGAGACGAAAGGT – 3′
BDTG 22 59.7 2 591 5′ -CAATTCTATCTGGAACCTTTCGTC – 3′ 5′-ACCGCTCAAGTTGTTTTCGT – 3′
BDTG 23 60 2 601 5′ -GGCATTCTACTCGCCTACGA – 3′ 5′ -GCATTGCCTTGGATTGAGAT – 3′
BDTG 24 CHARGED 59.8 3 707 5′-TGCCTCGATGTTGTCCATTA – 3′ 5′ -TCAATGAGGTCCCATCAACA – 3′
BDTG 25 KINASE 60.1 4 & 5 1268 5′ -AGGGACAATTGGCTATGCAG – 3′ 5′ -AGAATTCAAGGCTCCCACCT – 3′
BDTG 26 Xa21(A1) LRR 59.8 1 280 5′ -TGTTGTTCTCTGCGCTGC – 3′ 5′ -CGTCCTGAGGAAGGATAGGTT –3′
BDTG 27 59.6 1 408 5′ -CATCGCTGGGCAACCTAT – 3′ 5′ -TTGGACACGACTTCAAATATGG – 3′
BDTG 28 59.6 1 397 5′-CCCAGATCCTATTTGGAACATC – 3′ 5′ -TGGAAACAGAATCAGGGAGG – 3′
BDTG 29 59.9 1 410 5′ -AGGTTGCAAATTTGGTGGAG – 3′ 5′ -GGAATGCTAAATATTTCAATGGGA – 3′
BDTG 30 60.2 1 391 5′ -TAGGGCAAATTCCCATTGAA – 3′ 5′ -AAAACACCATTGGTTGGCA – 3′
BDTG 31 59.9 1 405 5′ -CTTTCGTTCAACAGCTTCCAC – 3′ 5′ -CACCATCTTGACTATCAAATTCTCC – 3′
BDTG 32 59.9 1 563 5′ -CTTTCGTTCAACAGCTTCCAC – 3′ 5′ -CAATGAAAGGAGGTAGACATAAACAGT – 3′
BDTG 33 SNAP 60.2 2 215 5′ -ACTGTTTATGTCTACCTCCTTTCATTG – 3′ 5′ -AATAGATTTGCTACGGTCGAACA – 3′
BDTG 34 KINASE 59.7 3 363 5′ -TTTGTTATGGAATTCTAGTGTTGGAA – 3′ 5′ -CCAACATAACATCAGCATGTCTC – 3′
  1. Gene - Resistance genes from which they were designed; Protein - Protein coded by the DNA sequence amplified by the corresponding marker; Ann Temp – Annealing Temperature of the respective primer pair; Exon no. - Exon of the original gene from which primer pair was designed.