Figure 1

The effects of siRNAs on cell migration. The siRNAs identified in the first screening (black bars), non-target siRNA as a negative control (NT, dark gray bars), anti-paxillin siRNA as a positive control (Pxn, light gray bars) and the pVenus-N1 vector were introduced into NBT-L2b cells by conventional transfection. The migration speed of individual cells (N > 50) was analyzed by the ImageJ software with a manual tracking plug-in. The speeds of non-target siRNA-expressing cells and of cells expressing the other siRNAs were compared by the Mann-Whitney U-test (P < 0.05, NT versus each siRNA). siRNAs with inhibitory effects (A) and accelerating effects (B) on cell migration are shown. Positive and negative controls were measured together with 5 or 6 kinds of samples on one 24-well microplate in each experiment, therefore, the height of bars designated Pxn differs in the various histograms.