Differential expression of CXCR1 and commonly used reference genes in bovine milk somatic cells following experimental intramammary challenge

Table 1 Primers used in two PCR assays multiplying three fragments of YWHAZ and CXCR1 , respectively

Gene	Primer	Sequence (5’ → 3’)	Product size (bp)
YWHAZ ^a	Forward	GAGCAAAAGACGGAAGGTGCT
	Reverse 1	CCAAAAGAGACAGTACATCATTGCA	109
	Reverse 2	TCCGATGTCCACAATGTCAAGT	497
	Reverse 3	TCCCCACCAGGACATACCAA	909
CXCR1 ^b	Forward	TCCCTGTGAGATAAGCACTGAGACA
	Reverse 1	AGCGACCAATCCGGCTGTA	131
	Reverse 2	CGTTGTATTGGCACCCAGGTC	505
	Reverse 3	GTCCGTGGCGAAACTTCTGG	863

^aTyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide [NM_174814.2].
^bChemokine (C-X-C motif) receptor 1 [NM_174360.2].

ISSN: 2730-6844