Differential expression of CXCR1 and commonly used reference genes in bovine milk somatic cells following experimental intramammary challenge

Table 2 Gene, primer and amplicon information

Gene full name	NCBI Gene ID	Genbank Accession number		Sequence (5’ → 3’)	Genomic location	Product size (bp)	Ta (°C)
ACTB	280979	NM_173979.3	F^a	CCTCACGGAACGTGGTTACA	CDS^b	87	58
Actin, beta			R^a	TCCTTGATGTCACGCACAATTT	CDS
B2M	280729	NM_173893.3	F	AGACACCCACCAGAAGATGG	CDS	206	59
Beta-2-microglobulin			R	CGGCAGCTGTACTGATCCTT	CDS
CXCR1	281863	NM_174360.2	F	TCCCTGTGAGATAAGCACTGAGACAC	CDS	118	64
Chemokine (C-X-C motif) receptor 1			R	GCTGTATAAGATGACCAGCATCACCA	CDS
H2A	506900	NM_001205596.1	F	GTCGTGGCAAGCAAGGAG	CDS	182	60
Histone 2 alpha			R	GATCTCGGCCGTTAGGTACTC	CDS
HPRT1	281229	NM_001034035.2	F	TGCTGAGGATTTGGAGAAGG	CDS	154	58
Hypoxanthine phosphoribosyl-transferase I			R	CAACAGGTCGGCAAAGAACT	CDS
PPP1R11 ⁴	504846	NM_001100295.1	F	ACCATCAAACTTCGGAAACG	CDS	166	57
Protein phosphatase 1, regulatory (inhibitor) subunit 11			R	CCTCCTCTTCCTCGTCATCA	CDS
RPS15A	337888	NM_001037443.2	F	AATGTCCTGGCTGATGCTCT	CDS	218	59
Ribosomal protein S15a			R	GGGCTGATCACTCCACACTT	CDS
SDHA	281480	NM_174178.2	F	GCAGAACCTGATGCTTTGTG	CDS	185	60
Succinate dehydrogenase flavoprotein subunit A			R	CGTAGGAGAGCGTGTGCTT	CDS
TBP ⁵	516578	NM_001075742.1	F	AATGGCTGCTGTGTTCTCCT	3’UTR^c	214	60
TATA box binding protein			R	TGACGCTCTGGTGTTCTCTGT	3’UTR
UBC	444874	NM_001206307.1	F	AGTTCAGTCTTCGTTCTTCTGTG	5’UTR^d	88	58
Ubiquitin C			R	GGTTTTACCAGTGAGGGTCTT	CDS
YWHAZ	287022	NM_174814.2	F	GCATCCCACAGACTATTTCC	CDS	120	60
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide			R	GCAAAGACAATGACAGACCA	CDS

ISSN: 2730-6844