Differential expression of CXCR1 and commonly used reference genes in bovine milk somatic cells following experimental intramammary challenge

Table 3 qPCR information

Gene	Median Cq ^a	SD Cq ^b	Calibration curve ^c
Gene	Median Cq ^a	SD Cq ^b	slope	y intercept	E (%)	r ²
ACTB	22.5	0.125	-3.541	18.607	91.6	0.997
B2M	17.7	0.137	-3.426	13.756	95.8	0.998
CXCR1	24.3	0.077	-3.195	17.240	105.6	0.999
H2A	23.1	0.186	-3.370	19.463	98.0	0.996
HPRT1	25.4	0.140	-3.463	20.475	94.4	0.997
RPS15A	19.3	0.151	-3.433	15.213	95.6	0.999
SDHA	23.4	0.148	-3.534	19.385	91.8	0.999
UBC	22.5	0.122	-3.248	18.150	103.2	0.998
YWHAZ	22.8	0.097	-3.368	19.139	98.1	0.999

^aMedian of the quantification cycle (Cq) of all samples.
^bAverage standard deviation of the Cq values of replicate samples.
^cSlope, y intercept, PCR efficiency and correlation coefficient estimated by qPCR on a 4-fold serial dilution of pooled cDNA of all samples.

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