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Figure 1 | BMC Genetics

Figure 1

From: Immunolabelling of human metaphase chromosomes reveals the same banded distribution of histone H3 isoforms methylated at lysine 4 in primary lymphocytes and cultured cell lines

Figure 1

Immunostaining of H3K4me3 in metaphase chromosomes from human primary lymphocytes. A. Metaphase chromosome spread immunostained with rabbit antibody to H3K4me3 and fluorescein isothiocyanate (FITC) conjugated goat anti-rabbit (green stain); DNA was visualized with 4′,6-diamidino-2-phenylindole (DAPI, pseudocoloured red). The three panels show FITC + DAPI (left), FITC (centre) and DAPI (right). B. Immunostained karyotype constructed from the metaphase spread shown. C. Reverse DAPI (rDAPI) karyotype constructed from the same spread, shown in black to resemble conventional G-banding.

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