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Figure 1 | BMC Genetics

Figure 1

From: Targeted Recombinant Progeny: a design for ultra-high resolution mapping of Quantitative Trait Loci in crosses between inbred or pure lines

Figure 1

Construction of a TRP mapping population. We assume a QTN mapped to a known confidence interval bounded by markers MU and MD. Construction of the TRP mapping population begins with one or more G1 individuals heterozygous for alternative alleles at the QTN and for a large series of markers spanning the interval from MU to MD. The G1 individuals are selfed, generating a G2 population. The G2 population is genotyped for the markers MU and MD identifying a subset of individuals (the G2R population) that carry a recombinant chromosome in this region together with one of the parental haplotypes . The G2R individuals are genotyped for the full set of internal markers, identifying the point of recombination of their recombinant chromosome. They are selfed in turn to generate the G3 population. The G3 individuals are genotyped for one of the markers heterozygous in their G2R parent to identify the haplotypes transmitted by the G2R parent. G3 individuals carrying one or two recombinant haplotypes (75% of the total) form the G3R mapping population. Non-recombinant G3 individuals that carry only parental type haplotypes serve to correct for polygenic family effects

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BMC Genomic Data

ISSN: 2730-6844

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