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Table 3 Population size for TRP-F2 design by width of initial QTL and target QTL , polygenic effect absent 1

From: Targeted Recombinant Progeny: a design for ultra-high resolution mapping of Quantitative Trait Loci in crosses between inbred or pure lines

C s c N TG3 N G2 N TRP N F2 N TRP /N F2
0.20 2 0.100 1026 57 1083 3750 0.289
0.20 4 0.050 2051 108 2159 7500 0.288
0.20 10 0.020 5129 261 5390 18750 0.287
0.10 2 0.050 1026 108 1134 7500 0.151
0.10 10 0.010 5129 517 5646 37500 0.151
0.05 2 0.025 1026 211 1237 15000 0.082
0.05 5 0.010 2564 517 3081 37500 0.082
0.05 10 0.005 5129 1029 6158 75000 0.082
0.02 2 0.010 1026 517 1543 37500 0.041
0.02 4 0.005 2051 1046 3097 75000 0.041
0.02 10 0.002 5129 2564 7693 187500 0.041
  1. 1Abbreviations: C, the original QTL in Morgans; s, the reduction factor; c, target QTL in Morgans; NTG3, total size of G3 population; NG2, total size of G2 population. NTRP, total number required across G2 and G3 populations; NF2, population size for equivalent QTL width using an F2 design. Assumptions: polygenic effect absent; standardized allele substitution effect, d = 0.2; reproductive potential of the G2 generation, n = 50; and confidence level set at (1-α) = 95%