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Table 3 Population size for TRP-F2 design by width of initial QTL and target QTL , polygenic effect absent 1

From: Targeted Recombinant Progeny: a design for ultra-high resolution mapping of Quantitative Trait Loci in crosses between inbred or pure lines

C

s

c

N TG3

N G2

N TRP

N F2

N TRP /N F2

0.20

2

0.100

1026

57

1083

3750

0.289

0.20

4

0.050

2051

108

2159

7500

0.288

0.20

10

0.020

5129

261

5390

18750

0.287

0.10

2

0.050

1026

108

1134

7500

0.151

0.10

10

0.010

5129

517

5646

37500

0.151

0.05

2

0.025

1026

211

1237

15000

0.082

0.05

5

0.010

2564

517

3081

37500

0.082

0.05

10

0.005

5129

1029

6158

75000

0.082

0.02

2

0.010

1026

517

1543

37500

0.041

0.02

4

0.005

2051

1046

3097

75000

0.041

0.02

10

0.002

5129

2564

7693

187500

0.041

  1. 1Abbreviations: C, the original QTL in Morgans; s, the reduction factor; c, target QTL in Morgans; NTG3, total size of G3 population; NG2, total size of G2 population. NTRP, total number required across G2 and G3 populations; NF2, population size for equivalent QTL width using an F2 design. Assumptions: polygenic effect absent; standardized allele substitution effect, d = 0.2; reproductive potential of the G2 generation, n = 50; and confidence level set at (1-α) = 95%