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Table 4 Population size for TRP-F2 design, polygenic effect present 1

From: Targeted Recombinant Progeny: a design for ultra-high resolution mapping of Quantitative Trait Loci in crosses between inbred or pure lines

C s c N TG3 N G2 N TRP N F2 N TRP /N F2
0.20 2 0.100 3796 211 4007 3750 1.068
0.20 4 0.050 7591 400 7991 7500 1.065
0.20 10 0.020 18978 968 19946 18750 1.064
0.10 2 0.050 3796 400 4196 7500 0.559
0.10 10 0.010 18978 1917 20895 37500 0.557
0.05 2 0.025 3796 779 4575 15000 0.305
0.05 5 0.010 9489 1917 11406 37500 0.304
0.05 10 0.005 18978 3814 22793 75000 0.304
0.02 2 0.010 3796 1917 5713 37500 0.152
0.02 4 0.005 7591 3814 11406 75000 0.152
0.02 10 0.002 18978 9508 28486 187500 0.152
  1. 1Abbreviations: C, the original QTL in Morgans; s, the reduction factor; c, target QTL in Morgans; NTG3, total size of G3 population; NG2, total size of G2 population. NTRP, total number required across G2 and G3 populations; NF2, population size for equivalent QTL width using an F2 design. Assumptions: polygenic effect present; standardized allele substitution effect, d = 0.2; reproductive potential of the G2 generation, n = 50; and confidence level set at (1-α) = 95%