Fig. 2

Protein sequence relationships of garlic alliinases from cv. Japo and two complete alliinase sequences. The phylogenetic tree was inferred using the neighbor-joining method [50]. The optimal tree with a branch length sum of 0.03415794 is shown. The percentage of replicate trees in which the associated sequence variants (SVs) clustered together in the bootstrap test (1000 replicates) is shown next to the branches [51]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances. The evolutionary distances were computed using the Poisson correction method [52] and are represented as the number of amino acid substitutions per site. All ambiguous positions were removed for each sequence pair. There were a total of 487 positions in the final data set. Evolutionary analyses were conducted with MEGA6 [49]