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Table 1 Characteristics of 21 microsatellite loci used in present study

From: Genetic diversity and relationship of Indian cattle inferred from microsatellite and mitochondrial DNA markers

Primers Primer sequences (5′-3′) Forward label Set Annealing temperature Product size (bp) Total number of alleles
BM1824 F-gagcaaggtgtttttccaatc VIC 4 58 °C 176-196 11
  R-cattctccaactgcttccttg      
CSSM08 F-cttggtgttactagccctggg VIC 3 55 °C 182-200 8
  R-gatatatttgccagagattctgca      
CSSM33 F-cactgtgaatgcatgtgtgtgagc NED 5 58 °C 144-188 21
  R-cccatgataagagtgcagatgact      
CSSM66 F-acacaaatcctttctgccagctga FAM 4 60 °C 167-207 19
  R-aatttaatgcactgaggagcttgg      
ETH10 F-gttcaggactggccctgctaaca NED 1 58 °C 185-221 14
  R-cctccagcccactttctcttctc      
ETH225 F-gaacctgcctctcctgcattgg VIC 4 64 °C 134-162 13
  R-actctgcctgtggccaagtagg      
ETH3 F-gatcaccttgccactatttcct NED 4 57 °C 90-124 16
  R-acatgacagccagctgctact      
HEL09 F-cccattcagtcttcagaggt FAM 5 59 °C 140-182 17
  R-cacatccatgttctcaccac      
HEL5 F-gcaggatcacttgttaggga VIC 3 55 °C 137-195 25
  R-agacgttagtgtacattaac      
ILSTS06 F-tgtctgtatttctgctgtgg FAM 5 58 °C 275-303 14
  R-acacggaagcgatctaaacg      
ILSTS11 F-gcttgctacatggaaagtgc NED 1 58 °C 249-273 10
  R-ctaaaatgcagagccctacc      
ILSTS34 F-aagggtctaagtccactggc VIC 5 59 °C 138-212 37
  R-gacctggtttagcagagagc      
ILSTS33 F-tattagagtggctcagtgcc PET 3 55 °C 131-163 16
  R-atgcagacagttttagaggg      
INRA05 F-caatctgcatgaagtataaatat FAM 2 54 °C 130-148 9
  R-cttcaggcataccctacacc      
INRA35 F-atcctttgcagcctccacattg FAM 3 54 °C 80-142 24
  R-ttgtgctttatgacactatccg      
INRA63 F-atttgcacaagctaaatctaacc PET 2 54 °C 162-190 14
  R-aaaccacagaaatgcttggaag      
MM12 F-caagacaggtgtttcaatct PET 4 52 °C 88-134 21
  R-atcgactctggggatgatgt      
MM8 F-cccaaggacagaaaagact NED 2 55 °C 114-144 12
  R-ctcaagataagaccacacc      
TGLA122 F-ccctcctccaggtaaatcagc VIC 1 58 °C 133-179 20
  R-aatcacatggcaaataagtacatac      
TGLA227 F-cgaattccaaatctgttaatttgct PET 2 55 °C 67-119 17
  R-acagacagaaactcaatgaaagca      
TGLA53 F-gctttcagaaatagtttgcattca FAM 1 58 °C 142-184 21
  R-atcttcacatgatattacagcaga