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Fig. 5 | BMC Genetics

Fig. 5

From: Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samples

Fig. 5

Analysis of 384 loci in 98 individuals collected from Orpheus Island and Magnetic Island. a 48 and 50 individuals from the respective reefs were pooled and quantitatively genotyped using qHRM. The allele frequencies between the reefs were tightly conserved between the two populations, with a Pearson’s r of 0.93 and a slope of 0.97. b We further validated 19 SNPs by genotyping individual members of each pool. The Pearson correlation between known and pooled genotypes for these SNPs is 0.93 with a regression slope of 0.77

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