Fig. 4

Four examples of nuclear tRNAs and their tRFs in T. cancriformis. Examples of highly abundant nuclear tRFs were selected. The secondary structures of the four mature nuclear tRNAs and the nucleotide sequence alignments between these tRNAs and their tRFs are shown (also see Fig. 2 legend). Different tRNA genes but with the same anticodon sequences (tRNA gene subtypes) are shown with upper-case Roman characters (I–V). The white circle in the secondary structure indicates the nonconserved nucleotides among the tRNA subtypes. Highly redundant tRF reads (≥500 reads) were used for the sequence alignments. Also see Additional file 1: Figure S6 for another 12 examples of nuclear tRNAs and their tRFs in T. cancriformis