Fig. 6From: Precise mapping and dynamics of tRNA-derived fragments (tRFs) in the development of Triops cancriformis (tadpole shrimp)Northern blot analysis of two T. cancriformis nuclear tRFs. Expression of (a) nuclear tRFGly(GCC) and (b) nuclear tRFLys(CUU) was detected in the adult stage of T. cancriformis with a northern blotting analysis using probes specific to the most-enriched tRF for each anticodon. Total RNA (1 or 4 μg) isolated from the adult stage was used. Washing treatments were performed at either 45 or 55 °CBack to article page