Table 3 Characteristics of 34 polymorphic SSR markers developed in enset (Ta = annealing temperature)
Marker name | Forward primer sequence (5'–3') | Reverse primer sequence (5'–3') | Repeat motif | Size range (bp) | Ta (°C) |
|---|---|---|---|---|---|
Evg-01 | AGTCATTGTGCGCAGTTTCC | GGAGGACTCCATGTGGATGAG | (CTT)8 | 100–120 | 60 |
Evg-02 | GGAGAAGCATTTGAAGGTTCTTG | TTCGCATTTATCCCTGGCAC | (AG)12 | 118–153 | 55 |
Evg-03 | ACAGCATAAGCGAAATAGCAG | ACAGCATAAGCGAAATAGCAG | (AG)12 | 107–123 | 60 |
Evg-04 | GCCATCGAGAGCTAAGGGG | GGCAAGGCCGTAAGATCAAC | (AG)21 | 113–147 | 60 |
Evg-05 | AGTTGTCACCAATTGCACCG | CCATCCTCCACACATGCC | (GA)22 | 103–141 | 60 |
Evg-06 | CCGAAGTGCAACACCAGAG | TCGCTTTGCTCAACATCACC | (GAA)9 | 202–211 | 60 |
Evg-07 | GGTTGTCCTCAAGAACGTGG | TGATGCCTAATGCCTCTCCC | (GTG)9 | 73–94 | 60 |
Evg-08 | CCATCGACGCCTTAACAGAG | TGAACCTCGGGAGTGACATAAG | (GA)21 | 164–190 | 60 |
Evg-09 | GCCTTTCGTATGCTTGGTGG | ACGTTGTTGCCGACATTCTG | (GA)13 | 141–175 | 60 |
Evg-10 | CAGCCTGTGCAGCTAATCAC | CAGCAGTTGCAGATCGTGTC | (AG)21 | 191–210 | 60 |
Evg-11 | GGCCTAGTGACATGATGGTG | TGATGCTAGATTCAAAGTCAAGG | (AC)13 | 135–160 | 60 |
Evg-12 | TGCAACCCTTTGCTGCATTC | AGCATCATTCGCCATGGTTG | (TG)14 | 135–154 | 60 |
Evg-13 | CTTGAAAGCATTGCATGTGGC | TCACCACTGTAGACCTCAGC | (CA)14 | 189–229 | 60 |
Evg-14 | AACCAATCTGCCTGCATGTG | GCCAGTGATTGTTGAGGTGG | (TGA)8 | 153–159 | 60 |
Evg-15 | TCCTTTAGGTTATTTGGTTGCC | CCTTGGACATGCCTCACATC | (AG)15 | 110–134 | 55 |
Evg-16 | GGCTAGTCCAGTTGGAAAGAG | GTAATCACCTCTGCCTTCACC | (AG)13 | 109–117 | 60 |
Evg-17 | GCGTCTGGTATGCTCAACTG | TCGGGAATGATACAGAGGCG | (TCA)8 | 111–154 | 60 |
Evg-18 | TCACTCCGATGGAAGGGATG | TCTCCACCATTTTAGTTGGCAC | (GAG)7 | 181–188 | 60 |
Evg-19 | GGTATGAAAGCCACACCACC | AGTTCACCCACGCCTCAC | (GT)16 | 234–255 | 60 |
Evg-20 | TTGCTCTCTGCTACTGACGG | CCGGTAACTTGGTGGAAGTC | (CA)17 | 138–148 | 60 |
Evg-21 | CAGGCAACCACTGCGATATG | CAGTTGTCTCCCCAGGTGC | (CA)12 | 106–116 | 60 |
Evg-22 | CTATCCAGGAGCCCATCTCG | ACTCTTCTCTTCGCCTGTGG | (CA)15 | 88–94 | 60 |
Evg-23 | CCACCAAAGGGCTCCTCG | TCGGATTCTCCCGCTATTGG | (AC)13 | 129–143 | 60 |
Evg-24 | TTTTCGGACGGTCTCTGTGG | TTCTTCTGCTGGCGTTTGAG | (TTG)8 | 155–162 | 60 |
Evg-25 | CACGTTGATGTCGTTCCGTC | GAATCGCTTCAAGGCGTAGG | (CT)13 | 201–229 | 60 |
Evg-26 | AAGCCATTGATGACTCCCCG | CAGTTGCACGCAGAGAAAAC | (AC)12 | 110–139 | 60 |
Evg-27 | GCAATAGAATGGTACGGAGCG | TTTTGACTGTTCCGACGGTG | (AG)16 | 103–123 | 60 |
Evg-28 | AAGCCACGGAATCAGCAAAC | ACCCACTACCTTTCCCTAAGC | (AC)12 | 201–209 | 60 |
Evg-29 | GTTCGACTCGTCCAAGAAGG | ACTGTCTTAGTGATAGCCATGC | (AC)15 | 103–113 | 60 |
Evg-48 | TAATTCTTCCCACCGGGGTC | GACCACTTACTTTTTGCACGC | (TG)12 | 127–133 | 60 |
Evg-49 | TCCTGCACCCTCCATATTCC | TCTCTCTCTCTGATCTTCGTAGC | (GA)13 | 226–234 | 60 |
Evg-50 | ATCTTGAACGTGGGGAAGGG | TGATACCTGGTGAGGATGCG | (TG)13 | 162–188 | 60 |
Evg-51 | TGAATGAGTGGGGGATGCTG | AATGGATCGTTATCCAACGTG | (CAT)9 | 145–148 | 60 |
Evg-52 | TATGGGAAGGGGATCCACAC | CAAATGCCGATAGGGACAGC | (CA)13 | 212–231 | 60 |