Table 2 Polymorphic gene-specific markers derived from the HvMATE gene between Hamelin and Br2
From: A new allele of acid soil tolerance gene from a malting barley variety
Marker name | Product length (bp) | Annealing temperature (°C) | Polymorphic type | Primers |
|---|---|---|---|---|
HvMATE-21indel | 497 | 62 | Agarose | F: GCTAGGGCTTGAAAACTGTTTG |
R: GACGAACTGTACGATGATGATGC | ||||
D0 | 352 | 55 | Agarose | F: GCCACGCCTTACAGTAAAGAAC |
R: CCTAGCTATCTCAAGTTGGCTTAC | ||||
Cit7 | 312 | 67 | SSCP | F: GCAGCCAAGACCTTGAGAAAGC |
R: GCCTGAACTAGCCCGAGAAATG | ||||
Cit14 | 225 | 58 | SSCP | F: TCGGGTATTGGAGTTAGAAGGG |
R: CGGGCACATTTGATGCAAGGAT | ||||
Cit16 | 205 | 55 | SSCP | F: CCCGAGTTATGTCATTTTTCCTCTC |
R: GGGCCTGGTTGGGCCTTAT | ||||
Cit6 | 225 | 55 | SSCP | F: ACCTTCCGTGACATCTGCTCTA |
R: ATCGGTGAGTCCTGGAATAGTG | ||||
U5 | 491 | 55 | SSCP | F: CACACAACTGGAAAACAACTACC |
R: GGATAAAACTTCAGTGCGACG | ||||
Cit1501 | 332 | 55 | SSCP | F: GAAGGGGCCTATTGCTTCAC |
R: CACCCATAAGTTGTGGTTCGG | ||||
Cit19 | 355 | 67 | SSCP | F: TGGTGAAACGGGCATGTCTC |
R: GAAACCAGGTATATTGCAAGAGC | ||||
U12f + U11r | 726 | 55 | Agarose | F: TCGTCAATCGCAACTCTCAGA |
R: CATATCGTTTGTCGTATCACGC | ||||
U4 | 404 | 55 | SSCP | F: CAAGTGTGAAATAGAGAGTCGGTAG |
R: CGCAAGAACATTTTTGTCACG | ||||
D5 | 453 | 55 | SSCP | F: CGGGTATTGGAGTTAGAAGGG |
R: GCTATAAAGTCCACGCTATGCAG | ||||
D3 | 448 | 58 | SSCP | F: CTCCTGCGAGGCAGATGAG |
R: CTCGCTCTCCCTAATGGTGG | ||||
D01R | 362 | 60 | SSCP | F: GCTCAACCAGACTCAGGTAAGC |
R: CCAAACAGGGCCTAAGCTTC | ||||
D202rr | 474 | 60 | SSCP | F: GTCTTCAACAGCATGATTAAGGTC |
R: CAAACCTAGCACTATTCGGGTG | ||||
D4FF | 440 | 58 | SSCP | F: CAATCCTTGCATCAAATGTGC |
| Â | Â | Â | Â | R: GGCCCTAAGATAGAAGCACAAG |