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Fig. 5 | BMC Genetics

Fig. 5

From: Genome-wide evolutionary and functional analysis of the Equine Repetitive Element 1: an insertion in the myostatin promoter affects gene expression

Fig. 5

Model for ERE1 integration via retrotransposition at the myostatin promoter. a Sequence of the empty wild type myostatin locus, the coordinates of the locus in the horse genome reference sequence (equCab2.0) are indicated on both sides. The promoter elements located upstream the transcription start site are shown: CAAT-box (pink), E-boxes (green), TATA boxes (yellow). The black arrowhead points to the position of the single strand break. b Annealing through microhomology (TTTTT/AAAAA) of the ERE1 RNA (light blue) to the single stranded DNA end originated after the nick and reverse transcription. The cDNA produced by retrotranscription is shown in dark blue. c Cleavage of the second DNA strand (black arrowhead). d Synthesis of the second strand of ERE1 cDNA and gap filling. e Sequence of the ERE1+ myostatin locus: the ERE1 element is integrated (dark blue) and the target site is duplicated (boxed nucleotides)

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