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Fig. 6 | BMC Genetics

Fig. 6

From: Genome-wide evolutionary and functional analysis of the Equine Repetitive Element 1: an insertion in the myostatin promoter affects gene expression

Fig. 6

Reporter gene assay. The reporter gene assay (eGFP expression) was carried out in human HeLa cells (left) and in horse immortalized fibroblasts (right). a Fluorescence microscopy images of cells transfected with the two constructs containing the eGFP gene under the control of the ERE1- (top) and ERE1+ (bottom) promoter. DAPI-staineing is shown on the right of each image. b Western blots with anti-tubulin (loading control, top) and anti-eGFP (bottom) antibodies using protein extracts from cells transfected with the plasmid containing the ERE1- (left) and the ERE1+ (right) promoter. c Quantification of eGFP expression by quantitative RT-PCR. The expression levels of the eGFP transcript are indicated in arbitrary units. eGFP levels in cells transfected with the ERE1- plasmid were used as reference and set to 1.0. NTCs, no-template controls

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BMC Genomic Data

ISSN: 2730-6844

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