Fig. 3

FSHB promoter activity is dependent on ACVR2A expression in response to activin A in LβT2 cells. The pGL3-Basic-FSHB (chr2:107,059,651–107,061,641 on the GRCm38/mm10 mouse genome assembly) and pCAGGS-ACVR2A plasmids were cotransfected with pRL-TK into LβT2 cells. At 18 h post-transfection, recombinant activin A was added for 6 h, and the Firefly-to-Renilla luminescence ratios were measured. Bars represent the mean luminescence ± SEM obtained in triplicate from 3 independent experiments. P values were determined by Tukey–Kramer post-hoc test (P < 0.01)