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Fig. 3 | BMC Genetics

Fig. 3

From: The chromosomal constitution of fish hybrid lineage revealed by 5S rDNA FISH

Fig. 3

Mitotic metaphase spreads and karyotypes of RCC, 2 nF2 and 4 nF3 fish with FISH signals of 5S rDNA. (a) Two strong and a few weak signals were detected in RCC fish; the boxes indicated two major signals of 5S rDNA (green). (b) The karyotype of RCC fish revealed two strong signals located on the first pair of larger-sized SM chromosomes. (c) One strong signal and a few weak signals in 2nF2 fish (green),the box indicated the one major signal of 5S rDNA (green). (d) The karyotype of 2nF2 fish show one strong signal on the first group of larger-sized SM chromosomes. (e) The FISH analysis of 4nF3 fish exhibited the double signal feature of two strong and a few weak signals (green), the boxes indicated the two major signals (green). (f ) The karyotype of 4nF3 fish showed two strong signals on the first group of larger-sized SM chromosomes. All metaphase chromosomes were counterstained with DAPI and appear blue. (g) The chromosome pairing in Meiosis I of spermatocytes in 4nF3 showed 100 bivalents under light microscope; (h) Two signals of 5S rDNA located on a separating bivalent. M: metacentric; SM: submetacentric; ST subtelelocentric; T: telocentric. (a-f) Bar = 3 μm; (g,h) Bar = 5 μm

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